Molecular Cloning, Expression and Purification of G-CSF Isoform D, an Alternative Splice Variant of Human G-CSF
نویسندگان
چکیده
منابع مشابه
G-CSF and GM-CSF in Neutropenia.
G-CSF and GM-CSF are used widely to promote the production of granulocytes or APCs. The U.S. Food and Drug Administration approved G-CSF (filgrastim) for the treatment of congenital and acquired neutropenias and for mobilization of peripheral hematopoietic progenitor cells for stem cell transplantation. A polyethylene glycol-modified form of G-CSF is approved for the treatment of neutropenias. ...
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The macrophage and granulocyte colony-stimulating factors, M-CSF and G-CSF, act in vitro to induce proliferation and differentiation of monocyte and granulocyte progenitor cells, respectively. We show here that both of these CSFs can be produced by stimulated human blood monocytes, but the M-CSF and G-CSF genes are independently regulated. Recombinant human interleukin-3 (IL-3) and GM-CSF prima...
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Purified preparations of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte CSF (G-CSF), and interleukin 3 (IL-3 or multi-CSF) alone and in combination, have been compared for their stimulatory effects on human granulocyte-macrophage colony forming cells (GM-CFC). In cultures of unseparated normal human bone marrow, the combinations of G-CSF plus IL-3 and G...
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Various cytokines play roles in the process of granulocytic proliferation, differentiation and maturation from hemopoietic stem/progenitor cells and in the functional activation of mature granulocytes. Among these cytokines, the most essential is granulocyte colony-stimulating factor (G-CSF), which is a 19KD glycoprotein composed of 174 amino acids with one o-linked sugar chain. This molecule i...
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The hematopoietic growth factors granulocyte/macrophage colony-stimulating factor (GM-CSF) and G-CSF, available as recombinant products, stimulate the growth in culture of blasts from patients with acute myeloblastic leukemia (AML). We used cDNA probes for each gene to study the genomic organization in blast cells of 22 patients and expression in the blast cells of i 8 patients. Alteration in t...
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ژورنال
عنوان ژورنال: Iranian Journal of Allergy, Asthma and Immunology
سال: 2019
ISSN: 1735-5249,1735-1502
DOI: 10.18502/ijaai.v18i4.1420